| Long name: |
CHK2 (ser33) Primary Polyclonal Antibody |
| Also known as: |
CHK2 (ser33) PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
CHK2 ser33 |
| Specificity: |
This is a highly specific antibody against CHK2 ser33 |
| Modification(s): |
Phosphorylation |
| Modification site(s): |
ser33 |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of ser33 |
| Gene ID number: |
11200 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Chk2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, Chk2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases, Indeed, Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block, Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif), The SQ/TQ cluster domain, The amino-terminal domain of Chk2 contains a series of seven serine and threonine residues (Ser19, These are known to be preferred sites for phosphorylation by ATM/ATR kinases, This kinase may have a role in meiosis as well, Thr26, Thr68 and other sites in this region become phosphorylated by ATM/ATR, UV irradiation or hydroxyurea treatment, after DNA damage by ionizing radiation (IR), and may also participate in transduction of the DNA damage and replicational stress signals, preventing the entry into mitosis, seems to have a regulatory function, therefore, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain, Chk2 is a serine/threonine kinase involved in the control of cell cycle checkpoints |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
CDS 1, CDS1, CHK2 checkpoint homolog, CHK2 checkpoint homolog, CHK2 checkpoint homolog S, CHK2_HUMAN, Checkpoint kinase 2, Checkpoint like protein CHK2, Chek 2, Chek2, Chk 2, HuCds 1, HuCds1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2, Serine/threonine-protein kinase Chk2, pombe, bA444G7 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
CHK2 (ser33) |
| Short name: |
CHK2 (ser33) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CHK2 (ser33) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16627 |
| Gene: |
CHEK2 |
More about : CHEK2 |
| Long gene name: |
checkpoint kinase 2 |
| Synonyms gene: |
RAD53 |
| Synonyms gene name: |
CHK2 (checkpoint, S, pombe) , pombe) homolog CHK2 checkpoint homolog (S |
| Synonyms: |
CDS1 CHK2 HuCds1 PP1425 bA444G7 |
| Locus: |
22q12, 1 |
| Discovery year: |
2001-09-19 |
| GenBank acession: |
AF086904 |
| Entrez gene record: |
11200 |
| Pubmed identfication: |
9836640 10097108 |
| RefSeq identity: |
NM_001005735 |
| Havana BLAST/BLAT: |
OTTHUMG00000151023 |
| Locus Specific Databases: |
LRG_302 |