| Long name: |
Smad3 (Ser204) Primary Polyclonal Antibody |
| Also known as: |
Smad3 (Ser204) PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
Smad3 Ser204 |
| Specificity: |
This phosphorylation site is homologous to that of Ser204 in Mouse and Rat, This is a highly specific antibody against Smad3 Ser204 |
| Modification(s): |
Phosphorylation |
| Modification site(s): |
Ser204 |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
180-230/425 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser204 [AG(p-S)PN] |
| Gene ID number: |
4088 |
| Swiss Prot: |
P84022 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription, Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes, Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator, Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures, This effect on wound healing appears to be hormone-sensitive, activates transcription, on formation of the SMAD3/SMAD4 complex, Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
HSPC193, HsT17436, JV15-2, LDS1C, MAD homolog 3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, Smad3, hMAD-3, hSMAD3, LDS3 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Smad3 (Ser204) |
| Short name: |
Smad3 (Ser204) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
SMAD family member 3 (Ser204) (Antibody to) |
| Alternative technique: |
antibodies |