| Long name: |
AKT1+2+3 (Tyr315+316+312) Antibody |
| Also known as: |
AKT1+2+3 (Tyr315+316+312) Antibody |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
AKT1+2+3 Tyr315+316+312 |
| Specificity: |
AKT2 when phosphorylated at Tyr316 in Human, Mouse, Mouse, Mouse, Rat, Rat, Rat or Bovine, This antibody will detect AKT 1 when phosphorylated at Tyr315 in Human, and AKT3 when phosphorylated at Tyr312 in Human, or Bovine, or Bovine, This is a highly specific antibody against AKT1+2+3 Tyr315+316+312 |
| Modification(s): |
Phosphorylation |
| Modification site(s): |
Tyr315+316+312 |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
280-330/480 |
| Subcellular location: |
Cell membrane, Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr315 |
| Gene ID number: |
207, 208, 10000 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus, AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, AKT was originally cloned from the retrovirus AKT8, AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform, Each isoform has a pleckstrin homology (PH)domain, Its tight control over cell proliferation and cell viability are manifold, Once phosphorylated, PI3 kinase recruits AKT to the membrane, There are three mammalian isoforms of Akt: AKT1 (PKB alpha), a kinase domain and a carboxy terminal regulatory domain, also known as protein kinase B (PKB), and is a key regulator of many signal transduction pathways, cytokines and insulin), is a 57 kDa serine/threonine protein kinase, overexpression or inappropriate activation of AKT has been seen in many types of cancer, where it is activated by PDK1 phosphorylation, AKT |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
AKT, AKT 1, AKT 2, AKT 3, AKT-1, AKT-2, AKT-3, AKT1, AKT1/2/3 phospho Y315 + Y316 + Y312, AKT1/AKT2/AKT3Tyr315/316/312, AKT1_HUMAN, AKT2, AKT2_HUMAN, AKT3, AKT3_HUMAN, C AKT, MGC9965, MGC99656, Oncogene AKT1, PKB, PKB alpha, PKB beta, PKB gamma, PKB-ALPHA, PRKBA, Protein Kinase B Alpha, Protein kinase B, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC PK Alpha, RAC Serine/Threonine Protein Kinase, RAC alpha serine/threonine protein kinase, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, Rac protein kinase alpha, cAKT, v akt murine thymoma viral oncogene homolog 1, vAKT Murine Thymoma Viral Oncogene Homolog 1, AKT1/AKT2/AKT3 Tyr315+Tyr316+Tyr312 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
AKT1+2+3 (Tyr315+316+312) |
| Short name: |
AKT1+2+3 (Tyr315+316+312) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
v-akt murine thymoma viral oncogene homolog 1+2+3 (Tyr315+316+312) (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, AKT1 and IDBG-635767 and ENSBTAG00000017636 and, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, nuclei, phosphatidylinositol-3, this GO :0000060 and protein import into nucleus, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004712 : protein serine/threonine/tyrosine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005080 : protein kinase C binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0005547 : phosphatidylinositol-3, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004712 : protein serine/threonine/tyrosine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005080 : protein kinase C binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0005547 : phosphatidylinositol-3, this GO :0016301 : kinase activity, this GO :0016772 : transferase activity, this GO :0019899 : enzyme binding, this GO :0019901 : protein kinase binding, this GO :0030235 : nitric-oxide synthase regulator activity, this GO :0042802 : identical protein binding, this GO :0043325 : phosphatidylinositol-3, this GO :0071889 : 14-3-3 protein binding, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0019899 : enzyme binding and also this GO :0019901 : protein kinase binding and also this GO :0030235 : nitric-oxide synthase regulator activity and also this GO :0042802 : identical protein binding and also this GO :0043325 : phosphatidylinositol-3, transferring phosphorus-containing groups and molecular function this GO :0018105 and peptidyl-serine phosphorylation and biological process this GO :0019899 and enzyme binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0030030 and cell projection organization and biological process this GO :0030154 and cell differentiation and biological process this GO :0030163 and protein catabolic process and biological process this GO :0030168 and platelet activation and biological process this GO :0030212 and hyaluronan metabolic process and biological process this GO :0030235 and nitric-oxide synthase regulator activity and molecular function this GO :0030307 and positive regulation of cell growth and biological process this GO :0030334 and regulation of cell migration and biological process this GO :0031018 and endocrine pancreas development and biological process this GO :0031295 and T cell costimulation and biological process this GO :0031659 and positive regulation of cyclin-dependent protein kinase activity involved in G1/S and biological process this GO :0031999 and negative regulation of fatty acid beta-oxidation and biological process this GO :0032094 and response to food and biological process this GO :0032270 and positive regulation of cellular protein metabolic process and biological process this GO :0032287 and peripheral nervous system myelin maintenance and biological process this GO :0032436 and positive regulation of proteasomal ubiquitin-dependent protein catabolic process and biological process this GO :0032869 and cellular response to insulin stimulus and biological process this GO :0032880 and regulation of protein localization and biological process this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0034405 and response to fluid shear stress and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0036064 and ciliary basal body and cellular component this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042593 and glucose homeostasis and biological process this GO :0042640 and anagen and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043154 and negative regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0043325 and phosphatidylinositol-3, translocation and biological process this GO :0001649 and osteoblast differentiation and biological process this GO :0001893 and maternal placenta development and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0001938 and positive regulation of endothelial cell proliferation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004712 and protein serine/threonine/tyrosine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005080 and protein kinase C binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005547 and phosphatidylinositol-3, 4, 4, 4, 4-bisphosphate binding, 4-bisphosphate binding and also this GO :0071889 : 14-3-3 protein binding, 4-bisphosphate binding and molecular function this GO :0043491 and protein kinase B signaling and biological process this GO :0043536 and positive regulation of blood vessel endothelial cell migration and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045429 and positive regulation of nitric oxide biosynthetic process and biological process this GO :0045600 and positive regulation of fat cell differentiation and biological process this GO :0045725 and positive regulation of glycogen biosynthetic process and biological process this GO :0045792 and negative regulation of cell size and biological process this GO :0045861 and negative regulation of proteolysis and biological process this GO :0045907 and positive regulation of vasoconstriction and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046209 and nitric oxide metabolic process and biological process this GO :0046326 and positive regulation of glucose import and biological process this GO :0046329 and negative regulation of JNK cascade and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0046889 and positive regulation of lipid biosynthetic process and biological process this GO :0048009 and insulin-like growth factor receptor signaling pathway and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0050999 and regulation of nitric-oxide synthase activity and biological process this GO :0051000 and positive regulation of nitric-oxide synthase activity and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0051146 and striated muscle cell differentiation and biological process this GO :0060644 and mammary gland epithelial cell differentiation and biological process this GO :0060709 and glycogen cell differentiation involved in embryonic placenta development and biological process this GO :0060716 and labyrinthine layer blood vessel development and biological process this GO :0061024 and membrane organization and biological process this GO :0070141 and response to UV-A and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process this GO :0071363 and cellular response to growth factor stimulus and biological process this GO :0071364 and cellular response to epidermal growth factor stimulus and biological process this GO :0071407 and cellular response to organic cyclic compound and biological process this GO :0071456 and cellular response to hypoxia and biological process this GO :0071889 and 14-3-3 protein binding and molecular function this GO :0090004 and positive regulation of establishment of protein localization to plasma membrane and biological process this GO :0090201 and negative regulation of release of cytochrome c from mitochondria and biological process this GO :0097011 and cellular response to granulocyte macrophage colony-stimulating factor stimulus and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :0097194 and execution phase of apoptosis and biological process this GO :1900740 and positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway and biological process this GO :1901653 and cellular response to peptide and biological process this GO :1901976 and regulation of cell cycle checkpoint and biological process this GO :1902176 and negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway and biological process this GO :2001240 and negative regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, 5-trisphosphate binding, 5-trisphosphate binding and also this GO :0016301 : kinase activity and also this GO :0016772 : transferase activity, 5-trisphosphate binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005819 and spindle and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0005977 and glycogen metabolic process and biological process this GO :0005978 and glycogen biosynthetic process and biological process this GO :0005979 and regulation of glycogen biosynthetic process and biological process this GO :0006006 and glucose metabolic process and biological process this GO :0006412 and translation and biological process this GO :0006417 and regulation of translation and biological process this GO :0006464 and cellular protein modification process and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006469 and negative regulation of protein kinase activity and biological process this GO :0006809 and nitric oxide biosynthetic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0006924 and activation-induced cell death of T cells and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007281 and germ cell development and biological process this GO :0007568 and aging and biological process this GO :0007596 and blood coagulation and biological process this GO :0008283 and cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0008637 and apoptotic mitochondrial changes and biological process this GO :0009408 and response to heat and biological process this GO :0009725 and response to hormone and biological process this GO :0010467 and gene expression and biological process this GO :0010507 and negative regulation of autophagy and biological process this GO :0010748 and negative regulation of plasma membrane long-chain fatty acid transport and biological process this GO :0010765 and positive regulation of sodium ion transport and biological process this GO :0010907 and positive regulation of glucose metabolic process and biological process this GO :0010951 and negative regulation of endopeptidase activity and biological process this GO :0010975 and regulation of neuron projection development and biological process this GO :0015630 and microtubule cytoskeleton and cellular component this GO :0015758 and glucose transport and biological process this GO :0016070 and RNA metabolic process and biological process this GO :0016071 and mRNA metabolic process and biological process this GO :0016301 and kinase activity and molecular function this GO :0016310 and phosphorylation and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0016772 and transferase activity, v-akt murine thymoma viral oncogene homolog 1 |
| Identity: |
391 |
| Gene: |
AKT1 |
More about : AKT1 |
| Long gene name: |
AKT serine/threonine kinase 1 |
| Synonyms gene name: |
v-akt murine thymoma viral oncogene homolog 1 |
| Synonyms: |
RAC PKB PRKBA AKT |
| Locus: |
14q32, 33 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
M63167 |
| Entrez gene record: |
207 |
| RefSeq identity: |
NM_005163 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000170795 |
| Locus Specific Databases: |
LRG_721 |