| Long name: |
Biotin Conjugated, Collagen IV Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Collagen IV |
| Specificity: |
This is a highly specific antibody against Collagen IV |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1620-1665/1669 |
| Subcellular location: |
Secreted |
| Source: |
KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain |
| Gene ID number: |
1282 |
| Swiss Prot: |
P02462 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Arresten, Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation, Ligand for alpha1/beta1 integrin, Specifically inhibits endothelial cell proliferation, The C-terminal half is found to possess the anti-angiogenic activity, comprising the C-terminal NC1 domain, forming a 'chicken-wire' meshwork together with laminins, inhibits angiogenesis and tumor formation, migration and tube formation, proteoglycans and entactin/nidogen, Type IV collagen is the major structural component of glomerular basement membranes (GBM) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
COL4A1, Collagen alpha-1(IV) chain, HANAC, POREN1, arresten, ICH |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Collagen IV Conjugated |
| Short name: |
Conjugated, Collagen IV Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, Collagen IV (Antibody to) |
| Alternative technique: |
antibodies |