| Long name: |
FITC Conjugated, PDIA2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PDIA2 |
| Specificity: |
This is a highly specific antibody against PDIA2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human PDIA2 |
| Gene ID number: |
64714 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
PDI, Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively), The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER, This retention is reported to be mediated by a KDEL receptor, as well as the reduction of disulphide bonds in proteins, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds, which catalyses disulphide interchange between thiols and protein dilsulphides, The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
PDA2 PDI PDIR |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PDIA2 Conjugated |
| Short name: |
FITC Conjugated, PDIA2 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, member 2 (Antibody to), protein disulfide isomerase family A |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BT, PDIA2 and IDBG-7384 and ENSG00000185615 and 64714, Pdia2 and IDBG-154856 and ENSMUSG00000024184 and 69191, member 2, multiple, peptidyl-proline 4-dioxygenase activity, this GO :0003756 and protein disulfide isomerase activity and molecular function this GO :0005496 and steroid binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0006457 and protein folding and biological process this GO :0006621 and protein retention in ER lumen and biological process this GO :0008152 and metabolic process and biological process this GO :0016209 and antioxidant activity and molecular function this GO :0016491 and oxidoreductase activity and molecular function this GO :0016853 and isomerase activity and molecular function this GO :0019511 and peptidyl-proline hydroxylation and biological process this GO :0031545 and peptidyl-proline 4-dioxygenase activity and molecular function this GO :0034976 and response to endoplasmic reticulum stress and biological process this GO :0045454 and cell redox homeostasis and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :1902175 and regulation of oxidative stress-induced intrinsic apoptotic signaling pathway and biological process, this GO :0003756 : protein disulfide isomerase activity, this GO :0003756 : protein disulfide isomerase activity and also this GO :0005496 : steroid binding and also this GO :0005515 : protein binding and also this GO :0016209 : antioxidant activity and also this GO :0016491 : oxidoreductase activity and also this GO :0016853 : isomerase activity and also this GO :0031545 : peptidyl-proline 4-dioxygenase activity, this GO :0005496 : steroid binding, this GO :0005515 : protein binding, this GO :0016209 : antioxidant activity, this GO :0016491 : oxidoreductase activity, this GO :0016853 : isomerase activity, this GO :0031545 : peptidyl-proline 4-dioxygenase activity, 51496 and IDBG-633666 and ENSBTAG00000016575 and 504356, protein disulfide isomerase family A |
| Identity: |
14180 |
| Gene: |
PDIA2 |
More about : PDIA2 |
| Long gene name: |
protein disulfide isomerase family A member 2 |
| Synonyms gene: |
PDIP |
| Synonyms gene name: |
member 2 , pancreatic protein disulfide isomerase-associated 2 protein disulfide isomerase family A, protein disulfide isomerase |
| Locus: |
16p13, 3 |
| Discovery year: |
2000-12-21 |
| GenBank acession: |
U19948 |
| Entrez gene record: |
64714 |
| Pubmed identfication: |
8561901 |
| RefSeq identity: |
NM_006849 |
| Classification: |
Protein disulfide isomerases |
| Havana BLAST/BLAT: |
OTTHUMG00000064891 |