| Long name: |
FITC Conjugated, AMPK alpha-1/2 (Thr183/Thr172) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
AMPK alpha-1/2 Thr183/Thr172 |
| Specificity: |
This is a highly specific antibody against AMPK alpha-1/2 Thr183/Thr172 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr172/Thr183 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from mouse AMPK alpha 2 around the phosphorylation site of Thr172 |
| Gene ID number: |
5562 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
AMPK is a cellular energy sensor conserved in all eukaryotic cells, AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation, Alternatively spliced transcript variants encoding distinct isoforms have been observed, It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK), It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways, The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio, [provided by RefSeq], The protein encoded by this gene belongs to the ser/thr protein kinase family |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
AMPK AMPKa2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
AMPK alpha-1/2 (Thr183/Thr172) Conjugated |
| Short name: |
FITC Conjugated, AMPK alpha-1/2 (Thr183/Thr172) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, AMPK a-1/2 (Thr183/Thr172) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9377 |
| Gene: |
PRKAA2 |
More about : PRKAA2 |
| Long gene name: |
protein kinase AMP-activated catalytic subunit alpha 2 |
| Synonyms gene: |
PRKAA |
| Synonyms gene name: |
AMP-activated, alpha 2 catalytic subunit , protein kinase |
| Locus: |
1p32, 2 |
| Discovery year: |
1995-12-12 |
| GenBank acession: |
BC069823 |
| Entrez gene record: |
5563 |
| Pubmed identfication: |
7959015 |
| RefSeq identity: |
NM_006252 |
| Havana BLAST/BLAT: |
OTTHUMG00000008282 |