| Long name: |
FITC Conjugated, ENO3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ENO3 |
| Specificity: |
This is a highly specific antibody against ENO3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
400-434/434 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human ENO3 |
| Gene ID number: |
2027 |
| Swiss Prot: |
P13929 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A switch from alpha enolase to beta enolase occurs in muscle tissue during development and ENO3, Mutations can result in decreased stability of the enzyme and be associated with a glycogen storage myopathy, This results in exercise-induced myalgias, a homodimer, generalized muscle weakness and fatigability, is found in skeletal muscle cells in the adult and appears to have a function in striated muscle development and regeneration, one of the three enolase isoenzymes found in mammals, ENO3 is also known as beta enolase |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ENO3 Conjugated |
| Short name: |
FITC Conjugated, ENO3 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, ENO3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3354 |
| Gene: |
ENO3 |
More about : ENO3 |
| Long gene name: |
enolase 3 |
| Synonyms gene name: |
(beta, enolase 3, muscle) , muscle) enolase 3 (beta |
| Synonyms name: |
beta-enolase muscle enriched enolase |
| Locus: |
17p13, 2 |
| Discovery year: |
1990-03-21 |
| GenBank acession: |
X16504 |
| Entrez gene record: |
2027 |
| Havana BLAST/BLAT: |
OTTHUMG00000099394 |