| Long name: |
FITC Conjugated, PKA gamma Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PKA gamma |
| Specificity: |
This is a highly specific antibody against PKA gamma |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human PKA gamma |
| Gene ID number: |
5568 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
C beta, C subunit is activated through autophosphorylation and direct phosphorylation at Thr197 by PDK-1, Certain amino acid substitutions make C gamma a distinct member of the cAMP dependent subfamily of protein kinases, PKA is comprised of two dimers of two subunits, PKA mediates diverse cellular mechanisms, R (regulatory) and C (catalytic), Tissue specific expression of C gamma, Two families of R subunit (RI and RII) and three C subunit isoforms (C alpha, When activated by the second messenger cAMP, acting to modulate it in a functionally specific way, and C gamma) have been identified each possessing distinct cAMP binding properties and resulting in different phosphorylation states, and suggest that C gamma may be distinct in its protein substrate specificity or its interaction with the different regulatory subunits, including proliferation, indicates pressure on C gamma during evolution, ion transport, plus gene transcription, regulation of metabolism, PKA (or cAPK) is a cyclic AMP dependent protein kinase |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
KAPCG_HUMAN, PKA C gamma, PRKACG, Protein kinase cAMP dependent catalytic gamma, Serine threonine protein kinase, KAPG |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PKA gamma Conjugated |
| Short name: |
FITC Conjugated, PKA gamma Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, PKA g (Antibody to) |
| Alternative technique: |
antibodies |