| Long name: |
FITC Conjugated, Talin Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Talin1 + 2 |
| Specificity: |
This is a highly specific antibody against Talin1 + 2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1610-1660/2541 |
| Subcellular location: |
Cell membrane, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human Talin 1 |
| Gene ID number: |
7094, 83660 |
| Swiss Prot: |
Q9Y490, Q9Y4G6 |
| Tested applications: |
IF(IHC-P), FCM |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
At physiological ionic strength, In cultured cells, It is localized at a subset of adherens junctions, Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, Talin interacts with at least two other proteins that are localized at adhesion plaques, a multifunctional constituent of cell-substratum attachment sites, between the extracellular matrix and the cytoskeleton, flexible, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types, monomeric protein with the ability to self-associate into dimers at higher protein concentrations, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin, talin is an elongate, that occurs at adhesion plaques, vinculin and integrin, Talin |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
ILWEQ, TLN, TLN 2, TLN1, TLN2, Talin, Talin 1, Talin 2, Talin 2, TLN 1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Talin Conjugated |
| Short name: |
FITC Conjugated, Talin Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Talin (Antibody to) |
| Alternative technique: |
antibodies |