| Long name: |
PRAS40 Primary Polyclonal Antibody |
| Also known as: |
PRAS40 PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
PRAS40 |
| Specificity: |
This is a highly specific antibody against PRAS40 |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
200-256/256 |
| Subcellular location: |
Cytoplasm |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human PRAS40 |
| Gene ID number: |
84335 |
| Swiss Prot: |
Q96B36 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins, Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis, Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases, Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, Inhibits RHEB-GTP-dependent mTORC1 activation, May also play a role in nerve growth factor-mediated neuroprotection, Substrate for AKT1 phosphorylation, Within mTORC1, but can also be activated by AKT1-independent mechanisms, mTORC1 is activated in response to growth factors or amino acids, mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E), mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals, which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation, Subunit of mTORC1 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
40 kDa proline-rich AKT substrate, AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, Lobe |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PRAS40 |
| Short name: |
PRAS40 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PRAS40 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
28426 |
| Gene: |
AKT1S1 |
More about : AKT1S1 |
| Long gene name: |
AKT1 substrate 1 |
| Synonyms gene name: |
AKT1 substrate 1 (proline-rich) |
| Synonyms: |
PRAS40 MGC2865 Lobe |
| Synonyms name: |
40 kDa , proline-rich Akt substrate |
| Locus: |
19q13, 33 |
| Discovery year: |
2004-03-04 |
| GenBank acession: |
BC022241 |
| Entrez gene record: |
84335 |
| Pubmed identfication: |
12524439 |
| RefSeq identity: |
NM_032375 |
| Classification: |
MTOR complex 1 |
| Havana BLAST/BLAT: |
OTTHUMG00000150246 |