| Long name: |
mTOR (Ser2481) Primary Polyclonal Antibody |
| Also known as: |
mTOR (Ser2481) PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
mTOR Ser2481 |
| Specificity: |
This phosphorylation site is homologous in the listed cross reactive species at the specified location, This is a highly specific antibody against mTOR Ser2481 |
| Modification(s): |
Phosphorylation |
| Modification site(s): |
Ser2481 |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
2450-2500/2549 |
| Subcellular location: |
Cell membrane, Lysosome, Mitochondrion, Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Ser2481 |
| Gene ID number: |
2475 |
| Swiss Prot: |
P42345 |
| Tested Applications: |
IF(IHC-P), IHC-P |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis, Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP, Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2), In parallel to protein synthesis, MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins, Moreover, Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor, Stimulates the pyrimidine biosynthesis pathway, This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E), To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A, Under nutrient sufficiency, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1, an allosteric activator of CAD at a later step in synthesis, and delayed regulation, and the inhibitor of translation initiation PDCD4, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, disrupting the interaction with AMPK and preventing activation of ULK1, energy and stress signals, eukaryotic translation initiation factor EIF4B, growth and survival in response to hormones, growth factors, mTORC1 also negatively regulates autophagy through phosphorylation of ULK1, nutrients, phosphorylates ULK1 at 'Ser-758', phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, this function is dependent on the mTORC1 complex, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), Serine/threonine protein kinase which is a central regulator of cellular metabolism |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, RAFT1, RAPT1, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, Serine/threonine-protein kinase mTOR, mTOR, FRAP |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
mTOR (Ser2481) |
| Short name: |
mTOR (Ser2481) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Mammalian target on rapamycin (Ser2481) (Antibody to) |
| Alternative technique: |
antibodies |