| Long name: |
Biotin Conjugated, eNOS (Ser1177) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
eNOS Ser1177 |
| Specificity: |
This is a highly specific antibody against eNOS Ser1177 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser1177 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Cell membrane, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser1177 |
| Gene ID number: |
4846 |
| Swiss Prot: |
P29474 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rabbit, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Isoform eNOS13C: Lacks eNOS activity, NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1, Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
Constitutive NOS, EC-NOS, ECNOS, Endothelial NOS, NOS type III, NOS3, NOSIII, Nitric oxide synthase, cNOS, endothelial, eNOS |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
eNOS (Ser1177) Conjugated |
| Short name: |
Conjugated, eNOS (Ser1177) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, eNOS (Ser1177) (Antibody to) |
| Alternative technique: |
antibodies |