| Long name: |
B-Raf (Ser444) Primary Polyclonal Antibody |
| Also known as: |
B-Raf (Ser444) PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
B-Raf Ser444 |
| Specificity: |
This is a highly specific antibody against B-Raf Ser444 |
| Modification(s): |
Phosphorylation |
| Modification site(s): |
Ser444 |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from mouse B-Raf around the phosphorylation site of Ser444 |
| Gene ID number: |
109880 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
1 BRAF is important for the regulation of cell proliferation and determination of cell fate during embryogenesis, B-Raf is a serine/threonine protein kinase that acts as a signal transducer from membrane-associated receptors to nuclear transcription factors, BRAF acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, Defects in BRAF are involved in a wide range of cancers, Interaction between Ras proteins and Raf proteins results in Raf-mediated phosphorylation and activation of MEK (also known as MAP kinase kinase), Raf protein family members, and differentiation in response to growth factors, and hormones, cytokines, have intrinsic serine/threonine kinase activity, including A Raf and B Raf, proliferation, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, The Raf kinases are important intermediates in signal transduction |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
B Raf proto oncogene serine threonine protein kinase, B raf 1, BRAF 1, BRAF1, BRAF_HUMAN, Braf, FLJ95109, MGC126806, MGC138284, Murine sarcoma viral v raf oncogene homolog B1, Murine sarcoma viral v-raf oncogene homolog B1, RAFB 1, RAFB1, cRmil, p94, v raf murine sarcoma viral oncogene homolog B1, 94 kDa B raf protein |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
B-Raf (Ser444) |
| Short name: |
B-Raf (Ser444) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
B-Raf (Ser444) (Antibody to) |
| Alternative technique: |
antibodies |