| Long name: |
FITC Conjugated, PKM2 (Tyr105) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PKM2 Tyr105 |
| Specificity: |
This is a highly specific antibody against PKM2 Tyr105 |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr105 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
97-121/531 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human PKM2 around the phosphorylation site of Tyr105 |
| Gene ID number: |
5315 |
| Swiss Prot: |
P14618 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Plays a general role in caspase independent cell death of tumor cells, Stimulates POU5F1-mediated transcriptional activation, The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production, The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival, generating ATP, Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
THBP1 OIP3 PK3 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PKM2 (Tyr105) Conjugated |
| Short name: |
FITC Conjugated, PKM2 (Tyr105) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, muscle (Tyr105) (Antibody to), pyruvate kinase |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
CTHBP and HEL-S-30 and OIP3 and PK3 and PKM2 and TCB and THBP1, PKM2 and IDBG-20117 and ENSG00000067225 and 5315, PKM2 and IDBG-645572 and ENSBTAG00000001601 and 512571, Pkm and IDBG-173276 and ENSMUSG00000032294 and 18746, muscle, nuclei, poly(A) RNA binding, this GO :0000287 and magnesium ion binding and molecular function this GO :0003824 and catalytic activity and molecular function this GO :0004743 and pyruvate kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005929 and cilium and cellular component this GO :0005975 and carbohydrate metabolic process and biological process this GO :0006006 and glucose metabolic process and biological process this GO :0006096 and glycolytic process and biological process this GO :0012501 and programmed cell death and biological process this GO :0016310 and phosphorylation and biological process this GO :0023026 and MHC class II protein complex binding and molecular function this GO :0030955 and potassium ion binding and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0003824 : catalytic activity and also this GO :0004743 : pyruvate kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0023026 : MHC class II protein complex binding and also this GO :0030955 : potassium ion binding and also this GO :0044822 : poly(A) RNA binding, this GO :0003824 : catalytic activity, this GO :0004743 : pyruvate kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0023026 : MHC class II protein complex binding, this GO :0030955 : potassium ion binding, this GO :0044822 : poly(A) RNA binding, pyruvate kinase |
| Identity: |
9021 |
| Gene: |
PKM |
More about : PKM |
| Long gene name: |
muscle , pyruvate kinase |
| Synonyms gene: |
PKM2 |
| Locus: |
15q23 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M23725 |
| Entrez gene record: |
5315 |
| Pubmed identfication: |
2040271 |
| Havana BLAST/BLAT: |
OTTHUMG00000172709 |