| Long name: |
Biotin Conjugated, MSK1 (Thr581) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MSK1 Thr581 |
| Specificity: |
This is a highly specific antibody against MSK1 Thr581 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr581 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Thr581 [LK(p-T)PC] |
| Gene ID number: |
9252 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ATF1 H3 and HMG14 in response to mitogen and stress, It is also known to be required for the phosphorylation of CREB, MSK1 autophosphorylate on at least 5 sites, MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase, MSK1 contains two kinase domains (N term and a C term), Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain, Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, Similar to RSK, MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
MSK1 RLPK |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MSK1 (Thr581) Conjugated |
| Short name: |
Conjugated, MSK1 (Thr581) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, MSK1 (Thr581) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10434 |
| Gene: |
RPS6KA5 |
More about : RPS6KA5 |
| Long gene name: |
ribosomal protein S6 kinase A5 |
| Synonyms gene name: |
90kD, polypeptide 5 , ribosomal protein S6 kinase |
| Locus: |
14q32, 11 |
| Discovery year: |
1999-03-10 |
| GenBank acession: |
AF074393 |
| Entrez gene record: |
9252 |
| Pubmed identfication: |
9687510 10702687 |
| RefSeq identity: |
NM_004755 |
| Classification: |
Mitogen-activated protein kinase-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000171055 |