| Long name: |
FITC Conjugated, Estrogen Receptor alpha (Ser104 + Ser106) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Estrogen Receptor alpha Ser104 + Ser106 |
| Specificity: |
These phosphorylation sites are homologous to Ser108 + Ser110 in Mouse and Ser109 + Ser111 in Rat, This is a highly specific antibody against Estrogen Receptor alpha Ser104 + Ser106 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser104 + Ser106 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
80-130/595 |
| Subcellular location: |
Cell membrane, Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human ER alpha around the phosphorylation site of Ser104/106 |
| Gene ID number: |
2099 |
| Swiss Prot: |
P03372 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ATF-2, Also mediates membrane-initiated estrogen signaling involving various kinase cascades, Can activate the transcriptional activity of TFF1, Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements, Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter, Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3, Isoform 3 can bind to ERE and inhibit isoform 1, Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production, Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor, Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components, Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner, Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences, Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP, Sp1 and Sp3, The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues, c-Fos, such as AP-1/c-Jun, the function involves CREBBP, to mediate ERE-independent signaling, Nuclear hormone receptor |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
ER-alpha, ESR, ESR1, ESRA, ESTRR, Era, Estradiol receptor, Estrogen receptor, NR3A1, Nuclear receptor subfamily 3 group A member 1, ER |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Estrogen Receptor alpha (Ser104 + Ser106) Conjugated |
| Short name: |
FITC Conjugated, Estrogen Receptor alpha (Ser104 + Ser106) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Estrogen Receptor a (Ser104 and Ser106) (Antibody to) |
| Alternative technique: |
antibodies |