| Long name: |
FITC Conjugated, CDK9 (Thr186) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
CDK9 Thr186 |
| Specificity: |
This is a highly specific antibody against CDK9 Thr186 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr186 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human CDK9 around the phosphorylation site of Thr186 |
| Gene ID number: |
1025 |
| Swiss Prot: |
P50750 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
H3 lysine 4 trimethylation (H3K4me3) and H3K36me3, In addition, MYOD1, Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), P-TEFb is also involved in cotranscriptional histone modification, Phosphorylates EP300, Promotes RNA synthesis in genetic programs for cell growth, Promotes cardiac myocyte enlargement, RPB1/POLR2A and AR, Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e, Replication stress response protein, SUPT5H and RDBP, TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling), The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro, This complex is inactive when in the 7SK snRNP complex form, also called positive transcription elongation factor b (P-TEFb), and the negative elongation factors DSIF and NELF, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, differentiation and viral pathogenesis, integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing, mRNA processing and mRNA export, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6, the CDK9/cyclin-K complex is required for genome integrity maintenance, thus reducing the breakdown of stalled replication forks and avoiding DNA damage, which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, Protein kinase involved in the regulation of transcription, g |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
PITALRE C-2k TAK |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
CDK9 (Thr186) Conjugated |
| Short name: |
FITC Conjugated, CDK9 (Thr186) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, CDK9 (Thr186) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1780 |
| Gene: |
CDK9 |
More about : CDK9 |
| Long gene name: |
cyclin dependent kinase 9 |
| Synonyms gene: |
CDC2L4 |
| Synonyms gene name: |
cyclin-dependent kinase 9 (CDC2-related kinase) |
| Locus: |
9q34, 11 |
| Discovery year: |
1998-04-29 |
| GenBank acession: |
L25676 |
| Entrez gene record: |
1025 |
| Pubmed identfication: |
8170997 9356449 |
| Classification: |
Cyclin dependent kinases P-TEFb complex |
| Havana BLAST/BLAT: |
OTTHUMG00000020715 |