| Long name: |
FITC Conjugated, RGS5 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
RGS5 |
| Specificity: |
This antibody may have a minor secondary cross-reactivity to the related proteins RGS3 and RGS4 based on a 67% and 73% non-sequential sequence similarity, respectively, This is a highly specific antibody against RGS5 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
80-130/181 |
| Subcellular location: |
Cell membrane, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from mouse RGS5 |
| Gene ID number: |
19737 |
| Swiss Prot: |
O08850 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Rat, Mouse |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha, Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
RGS5 Conjugated |
| Short name: |
FITC Conjugated, RGS5 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, RGS5 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10001 |
| Gene: |
RGS5 |
More about : RGS5 |
| Long gene name: |
regulator of G-protein signaling 5 |
| Synonyms gene name: |
regulator of G-protein signalling 5 |
| Locus: |
1q23, 3 |
| Discovery year: |
1998-10-12 |
| GenBank acession: |
AF030108 |
| Entrez gene record: |
8490 |
| Pubmed identfication: |
9747037 |
| RefSeq identity: |
NM_003617 |
| Classification: |
Regulators of G-protein signaling |
| Havana BLAST/BLAT: |
OTTHUMG00000034441 |