| Long name: |
FITC Conjugated, Ephrin B Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Ephrin B |
| Specificity: |
This is a highly specific antibody against Ephrin B |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Cytoplasm |
| Source: |
synthetic peptide from human Ephrin B conjugated to KLH |
| Gene ID number: |
1947 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact, Importantly, Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase, This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface, This reverse signaling event is thought to be critical for vessel maturation and neuronal development, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event, Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
EFNB1, EFNB2_HUMAN, ELK L, ELK ligand, ELKL, EPH related receptor tyrosine kinase ligand 2, Ephrin B Precursor, Ephrin B1, Ephrin B2, Ephrin-B, EphrinB, LERK 2, LERK2, CEK5 ligand |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Ephrin B Conjugated |
| Short name: |
FITC Conjugated, Ephrin B Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Ephrin B (Antibody to) |
| Alternative technique: |
antibodies |