| Long name: |
Biotin Conjugated, Ephrin B Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Ephrin B |
| Specificity: |
This is a highly specific antibody against Ephrin B |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Cytoplasm |
| Source: |
synthetic peptide from human Ephrin B conjugated to KLH |
| Gene ID number: |
1947 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact, Importantly, Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase, This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface, This reverse signaling event is thought to be critical for vessel maturation and neuronal development, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event, Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
EFNB1, EFNB2_HUMAN, ELK L, ELK ligand, ELKL, EPH related receptor tyrosine kinase ligand 2, Ephrin B Precursor, Ephrin B1, Ephrin B2, Ephrin-B, EphrinB, LERK 2, LERK2, CEK5 ligand |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Ephrin B Conjugated |
| Short name: |
Conjugated, Ephrin B Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, Ephrin B (Antibody to) |
| Alternative technique: |
antibodies |