| Long name: |
FITC Conjugated, Lymphocyte Activation Gene 3 Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Lymphocyte Activation Gene 3 |
| Specificity: |
This is a highly specific antibody against Lymphocyte Activation Gene 3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
210-260/525 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human Lymphocyte Activation Gene 3 |
| Gene ID number: |
297596 |
| Swiss Prot: |
Q5BK54 |
| Tested applications: |
IF(ICC), IF(IHC-P), FCM |
| Recommended dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
It is found on the cell surface of activated NK and T-lymphocytes, LAG-3 binds to HLA class-II antigens and is involved in lymphocyte activation, The sequence data, also known as CD223, and chromosomal localization all indicate a close relationship of LAG-3 to CD4, belongs to the immunoglobulin superfamily and contains 4 extracellular Ig-like domains, exon/intron organization, Lymphocyte-activation protein 3 (LAG-3) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
LAG-3, Lag3, Lymphocyte activation gene 3 protein, CD223 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Lymphocyte Activation Gene 3 Conjugated |
| Short name: |
FITC Conjugated, Lymphocyte Activation Gene 3 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Lymphocyte Activation Gene 3 (Antibody to) |
| Alternative technique: |
antibodies |