| Long name: |
FITC Conjugated, DC-SIGN Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
DC-SIGN |
| Specificity: |
This is a highly specific antibody against DC-SIGN |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
354-404/404 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human DC-SIGN |
| Gene ID number: |
30835 |
| Swiss Prot: |
Q9NNX6 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
HCV E2, HIV-2 gp120, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2, On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates, Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, SIV gp120, Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells, The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response, Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments, cytomegalovirus gB, dengue virus gE, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA, ebolavirus glycoproteins, including HIV-1 gp120, mannose in Klebsiella pneumonae LPS, Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
DC-SIGN CDSIGN DC-SIGN1 CLEC4L |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
DC-SIGN Conjugated |
| Short name: |
FITC Conjugated, DC-SIGN Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, DC-SIGN (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1641 |
| Gene: |
CD209 |
More about : CD209 |
| Long gene name: |
CD209 molecule |
| Synonyms gene name: |
CD209 antigen |
| Locus: |
19p13, 2 |
| Discovery year: |
2000-07-19 |
| GenBank acession: |
M98457 |
| Entrez gene record: |
30835 |
| Pubmed identfication: |
1518869 |
| RefSeq identity: |
NM_021155 |
| Classification: |
CD molecules Scavenger receptors C-type lectin domain family |
| Havana BLAST/BLAT: |
OTTHUMG00000182530 |