| Long name: |
Biotin Conjugated, Aurora A+B+C Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Aurora A+B+C |
| Specificity: |
This is a highly specific antibody against Aurora A+B+C |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
70-105/344 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human Aurora B |
| Gene ID number: |
9212 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A positive feedback between GSG2 and AURKB contributes to CPC localization, A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres, AURKB is also required for kinetochore localization of BUB1 and SGOL1, AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP, DES/desmin, GPAF, GSG2/Haspin, Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis, KIF2C, Key component of the cytokinesis checkpoint, Key regulator of active promoters in resting B- and T-lymphocytes, NSUN2, Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, Phosphorylation of INCENP leads to increased AURKB activity, Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, Phosphorylation of p53/TP53 negatively regulates its transcriptional activity, PubMed:24814515), RACGAP1, Required for central/midzone spindle assembly and cleavage furrow formation, SEPT1, The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly, VIM/vimentin, a complex that acts as a key regulator of mitosis, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, and histone H3, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, respectively), whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
AIM-1, ARK-1, ARK-2, ARK-3, Aurora 1, Aurora 3, Aurora kinase B, Aurora kinase C, Aurora- and IPL1-like midbody-associated protein 1, Aurora-related kinase 1, Aurora-related kinase 2, Aurora-related kinase 3, Aurora/IPL1-related kinase 1, Aurora/IPL1-related kinase 2, Aurora/IPL1-related kinase 3, Aurora/IPL1/Eg2 protein 2, Breast tumor-amplified kinase, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 13, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, Serine/threonine-protein kinase aurora-B, Serine/threonine-protein kinase aurora-C, hARK1, Aurora 2 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Aurora A+B+C Conjugated |
| Short name: |
Conjugated, Aurora A+B+C Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, Aurora A+B+C (Antibody to) |
| Alternative technique: |
antibodies |