| Long name: |
Biotin Conjugated, PDEF/SPDEF Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PDEF/SPDEF |
| Specificity: |
This is a highly specific antibody against PDEF/SPDEF |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human PDEF |
| Gene ID number: |
25803 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
It is also expressed in epithelial breast carcinoma, It is highly expressed in prostate carcinoma cells and is thought to be involved in the regulation of the prostate gland and/or prostate cancer development, It is thought to act as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter and also as a transcriptional activator for the SERPINB5 promoter, also known as PDEF is an ETS transcription factor expressed in the prostate epithelial cells, SPDEF |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
PDEF bA375E1, 3 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PDEF/SPDEF Conjugated |
| Short name: |
Conjugated, PDEF/SPDEF Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, PDEF/SPDEF (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
17257 |
| Gene: |
SPDEF |
More about : SPDEF |
| Long gene name: |
SAM pointed domain containing ETS transcription factor |
| Synonyms gene name: |
SAM pointed domain containing ets transcription factor |
| Locus: |
6p21, 31 |
| Discovery year: |
2003-11-27 |
| GenBank acession: |
AF071538 |
| Entrez gene record: |
25803 |
| Pubmed identfication: |
10625666 6857767 |
| RefSeq identity: |
NM_012391 |
| Classification: |
ETS transcription factor family |
| Havana BLAST/BLAT: |
OTTHUMG00000014548 |