| Long name: |
LEF-1 Primary Polyclonal Antibody |
| Also known as: |
LEF-1 PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
LEF-1 |
| Specificity: |
This is a highly specific antibody against LEF-1 |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
350-399/399 |
| Subcellular location: |
Nucleus |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human LEF-1 |
| Gene ID number: |
51176 |
| Swiss Prot: |
Q9UJU2 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Activates transcription of target genes in the presence of CTNNB1 and EP3, Binds DNA in a sequence-specific manner, Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells, Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling, Isoform 5 transcriptionally activates the fibronectin promoter, May play a role in hair cell differentiation and follicle morphogenesis, PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity), Regulates T-cell receptor alpha enhancer function, TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, Participates in the Wnt signaling pathway |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
LEF1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1, TCF1-alpha, TCF1ALPHA, TCF7L3, LEF-1 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
LEF-1 |
| Short name: |
LEF-1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
LEF-1 (Antibody to) |
| Alternative technique: |
antibodies |