| Long name: |
FITC Conjugated, E2F3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
E2F3 |
| Specificity: |
This is a highly specific antibody against E2F3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
420-465/465 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human E2F3 |
| Gene ID number: |
1871 |
| Swiss Prot: |
O00716 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication, E2F3 binds specifically to RB1 in a cell-cycle dependent manner, Inhibits adipogenesis, The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase, probably through the repression of CEBPA binding to its target gene promoters (By similarity), Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
E2F3 Conjugated |
| Short name: |
FITC Conjugated, E2F3 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, E2F3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3115 |
| Gene: |
E2F3 |
More about : E2F3 |
| Long gene name: |
E2F transcription factor 3 |
| Locus: |
6p22, 3 |
| Discovery year: |
1994-08-31 |
| GenBank acession: |
Y10479 |
| Entrez gene record: |
1871 |
| Pubmed identfication: |
8246996 |
| Classification: |
E2F transcription factors |
| Havana BLAST/BLAT: |
OTTHUMG00000016389 |