| Long name: |
FITC Conjugated, pan Cytokeratin Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
pan Cytokeratin |
| Specificity: |
This is a highly specific antibody against pan Cytokeratin |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
170-210/644 |
| Source: |
KLH conjugated synthetic peptide derived from human pan cytokeratin |
| Tested applications: |
IF(IHC-F), IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-F)(1:50-200), IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Pig, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
20 are epithelial genes and the remaining 10 are specific for trichocytes, Among them, Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, Cytokeratins interact with desmosomes and hemidesmosomes, In the cytoplasm, Numerous accessory proteins are involved in the genesis and maintenance of such structure, The cytokeratins are encoded by a family encompassing 30 genes, This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms, cell movement and differentiation, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
2, Cytokeratins, P-CK, [cytokeratins 1, pan CK, pan-CK, wide spectrum Cytokeratin, 4, 5, 6, 7, 8, 71, 72, 75, 78], pan-cytokeratin |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
pan Cytokeratin Conjugated |
| Short name: |
FITC Conjugated, pan Cytokeratin Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, pan Cytokeratin (Antibody to) |
| Alternative technique: |
antibodies |