| Long name: |
HRP Conjugated, DAPK1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
HRP Conjugated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
DAPK1 |
| Specificity: |
This is a highly specific antibody against DAPK1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1360-1410/1430 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human DAPK1 |
| Gene ID number: |
1612 |
| Swiss Prot: |
P53355 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke, Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-133 inducing injurious Ca(2+) influx through NMDA receptor channels, Isoform 2 cannot induce apoptosis but can induce membrane blebbing, MYL9 and DAPK3, Phosphorylates BECN1, Phosphorylates PIN1 resulting in inhibition of its catalytic activity, Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress, Phosphorylates RPS6, Phosphorylates STX1A and significantly decreases its binding to STXBP1, Phosphorylates TPM1, Phosphorylates TSC2, Regulates both type I apoptotic and type II autophagic cell deaths signal, Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition, The former is caspase-dependent, and autophagy, and cellular function, apoptosis, depending on the cellular setting, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway, enhancing stress fiber formation in endothelial cells, nuclear localization, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy, resulting in an irreversible neuronal death, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles, Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% Gentamicin, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
DAPK |
| Properties: |
Also monoclonal HRP conjugates are often used for Western Blot, HRP conjugates are often primary rabbit polyclonal antibodies couples to the , found in the roots of , (HRP), C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°, enzyme , horseradish, horseradish peroxidase |
| Gene target: |
DAPK1 Conjugated |
| Short name: |
Conjugated, DAPK1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
HRP |
| Alternative name: |
horseradish peroxidase coupled, death-associated protein kinase 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DAPK, DAPK1 and IDBG-636344 and ENSBTAG00000000738 and 540873, DAPK1 and IDBG-74918 and ENSG00000196730 and 1612, Dapk1 and IDBG-159357 and ENSMUSG00000021559 and 69635, Plasma membranes, alcohol group as acceptor, alcohol group as acceptor and also this GO :0042802 : identical protein binding, alcohol group as acceptor and molecular function this GO :0017148 and negative regulation of translation and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0071346 and cellular response to interferon-gamma and biological process this GO :0097190 and apoptotic signaling pathway and biological process this GO :1902042 and negative regulation of extrinsic apoptotic signaling pathway via death domain receptors and biological process this GO :2000310 and regulation of N-methyl-D-aspartate selective glutamate receptor activity and biological process, this GO :0003824 and catalytic activity and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004683 and calmodulin-dependent protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005516 and calmodulin binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0007165 and signal transduction and biological process this GO :0008625 and extrinsic apoptotic signaling pathway via death domain receptors and biological process this GO :0009103 and lipopolysaccharide biosynthetic process and biological process this GO :0010506 and regulation of autophagy and biological process this GO :0015629 and actin cytoskeleton and cellular component this GO :0016020 and membrane and cellular component this GO :0016772 and transferase activity, this GO :0003824 : catalytic activity, this GO :0003824 : catalytic activity and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004683 : calmodulin-dependent protein kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005516 : calmodulin binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004683 : calmodulin-dependent protein kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005516 : calmodulin binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0016773 : phosphotransferase activity, this GO :0042802 : identical protein binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0016773 : phosphotransferase activity, transferring phosphorus-containing groups and molecular function this GO :0016773 and phosphotransferase activity, death-associated protein kinase 1 |
| Identity: |
2674 |
| Gene: |
DAPK1 |
More about : DAPK1 |
| Long gene name: |
death associated protein kinase 1 |
| Synonyms gene name: |
death-associated protein kinase 1 |
| Locus: |
9q21, 33 |
| Discovery year: |
1995-04-27 |
| GenBank acession: |
X76104 |
| Entrez gene record: |
1612 |
| Pubmed identfication: |
8530096 |
| RefSeq identity: |
NM_004938 |
| Classification: |
Death associated protein kinases Ankyrin repeat domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000020150 |