| Long name: |
CEBP-alpha Primary Polyclonal Antibody |
| Also known as: |
CEBP-alpha PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
CEBP-alpha |
| Specificity: |
Due to the similarity in amino acid composition of this protein and the closely related CEBP-beta, this antibody may be cross-react with this protein due to a 77% non-sequential sequence similarity, This is a highly specific antibody against CEBP-alpha |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
250-300/358 |
| Subcellular location: |
Nucleus |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human CEBP-alpha |
| Gene ID number: |
1050 |
| Swiss Prot: |
P49715 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes (PubMed:11242107), Critical for the proper development of the liver and the lung (By similarity), Downregulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, During early embryogenesis, Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP), In adipose tissue, In liver, Necessary for terminal adipocyte differentiation, Proliferation arrest also depends on a functional binding to SWI/SNF complex (PubMed:14660596), Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters, To modulate lipogenesis, To regulate gluconeogenesis, To regulate these different processes at the proper moment and tissue, adipocytes, and cells of the lung and the placenta, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC, hepatocytes, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2, interplays with other transcription factors and modulators, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity), plays essential and redundant functions with CEBPB, regulates gluconeogenesis and lipogenesis through different mechanisms, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity), which is critical for its ability to induce adipocyte and granulocyte terminal differentiation, Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
C/EBP alpha, C/EBP-alpha, CCAAT/enhancer-binding protein alpha, CEBPA, CEBP |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
CEBP-alpha |
| Short name: |
CEBP-alpha Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CEBP-a (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1833 |
| Gene: |
CEBPA |
More about : CEBPA |
| Long gene name: |
CCAAT/enhancer binding protein alpha |
| Synonyms gene: |
CEBP |
| Synonyms gene name: |
CCAAT/enhancer binding protein (C/EBP), alpha |
| Synonyms: |
C/EBP-alpha |
| Locus: |
19q13, 11 |
| Discovery year: |
1991-11-27 |
| GenBank acession: |
M37197 |
| Entrez gene record: |
1050 |
| Pubmed identfication: |
1535333 1840554 |
| RefSeq identity: |
NM_004364 |
| Classification: |
CCAAT/enhancer binding proteins Basic leucine zipper proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000161461 |
| Locus Specific Databases: |
LRG_456 |