| Long name: |
FITC Conjugated, Calpain 2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Calpain 2 |
| Specificity: |
This is a highly specific antibody against Calpain 2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from mouse Calpain 2 |
| Gene ID number: |
824 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Both Calpain 1 and Calpain 2 are ubiquitously expressed, Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit, Domains in the large subunit include the amino terminal domain I, It is not clear that all calpains contain a small subunit, Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain, Other calpain family members (calpain 94, The calpain family members consist of a common small subunit (Calpain 4), The calpains appear to serve multiple physiological roles, The calpains have papain like activity, Whereas the 30 kDa subunit is shared by both enzymes, also known as Calpain 2, and a large variable subunit, and are countered by the endogenous calpain inhibitor, and ideas concerning the functions of these enzymes are in a state of rapid flux, and m calpain, and perhaps different functions, and the EF hand domain IV, are intracellular, calcium dependent cysteine proteases, calpastatin, domain III, etc) have more limited tissue distribution, ncl2, ncl3, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names, the proteinase domain II, thus the pain nomenclature, Calpain |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
CALP80, CALP80, CAN2_MOUSE, CANP 2, CANP L2, CANP2, CANPL 2, CANPL2, CANPml, CAPN 2, CAPN2, Calcium activated neutral proteinase, Calcium-activated neutral proteinase 2, Calpain 2, Calpain 2 catalytic subunit, Calpain 2 large catalytic subunit, Calpain 2 large subunit, Calpain 2 m/II large subunit, Calpain M type, Calpain M-type, Calpain large polypeptide L2, Calpain-2 catalytic subunit, Calpain-2 large subunit, Calpain2, Capa2, FLJ39928, M calpain, M calpin, M type, M-calpain, Millimolar calpain, Millimolar-calpain, large [catalytic] subunit, mCANP, Calcium activated neutral proteinase 2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Calpain 2 Conjugated |
| Short name: |
FITC Conjugated, Calpain 2 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Calpain 2 (Antibody to) |
| Alternative technique: |
antibodies |