| Long name: |
FITC Conjugated, Complement C4 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Complement C4 |
| Specificity: |
This is a highly specific antibody against Complement C4 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
70-120/1744 |
| Subcellular location: |
Secreted |
| Source: |
KLH conjugated synthetic peptide derived from human Complement C4 |
| Gene ID number: |
720721 |
| Swiss Prot: |
P0C0L4 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, C4a anaphylatoxin is a mediator of local inflammatory process, Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes, Derived from proteolytic degradation of complement C4, It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens, Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C4A, C4A2, C4A3, C4A4, C4A6, C4AD, C4B1, C4B12, C4B2, C4B3, C4B5, C4BD, C4B_2, C4F, C4S, CH, CO4, CPAMD2, CPAMD3, Complement C4-A, RG, C4 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Complement C4 Conjugated |
| Short name: |
FITC Conjugated, Complement C4 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Complement C4 (Antibody to) |
| Alternative technique: |
antibodies |