| Long name: |
Biotin Conjugated, Complement C4 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Complement C4 |
| Specificity: |
This is a highly specific antibody against Complement C4 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
70-120/1744 |
| Subcellular location: |
Secreted |
| Source: |
KLH conjugated synthetic peptide derived from human Complement C4 |
| Gene ID number: |
720721 |
| Swiss Prot: |
P0C0L4 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, C4a anaphylatoxin is a mediator of local inflammatory process, Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes, Derived from proteolytic degradation of complement C4, It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens, Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C4A, C4A2, C4A3, C4A4, C4A6, C4AD, C4B1, C4B12, C4B2, C4B3, C4B5, C4BD, C4B_2, C4F, C4S, CH, CO4, CPAMD2, CPAMD3, Complement C4-A, RG, C4 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Complement C4 Conjugated |
| Short name: |
Conjugated, Complement C4 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, Complement C4 (Antibody to) |
| Alternative technique: |
antibodies |