| Long name: |
FITC Conjugated, PDI Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PDI |
| Specificity: |
This is a highly specific antibody against PDI |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human PDI |
| Gene ID number: |
2923 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
PDI, Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively), The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER, This retention is reported to be mediated by a KDEL receptor, as well as the reduction of disulphide bonds in proteins, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds, which catalyses disulphide interchange between thiols and protein dilsulphides, The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
HPAD10 PDI1 PDI PAD1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PDI Conjugated |
| Short name: |
FITC Conjugated, PDI Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, PDI (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
18367 |
| Gene: |
PADI1 |
More about : PADI1 |
| Long gene name: |
peptidyl arginine deiminase 1 |
| Synonyms gene name: |
peptidyl arginine deiminase, type I |
| Synonyms name: |
peptidylarginine deiminase type I protein-arginine deiminase type-1 hPAD-colony 10 |
| Locus: |
1p36, 13 |
| Discovery year: |
2002-03-12 |
| GenBank acession: |
AB033768 |
| Entrez gene record: |
29943 |
| Pubmed identfication: |
12416996 |
| RefSeq identity: |
NM_013358 |
| Classification: |
Peptidyl arginine deiminases |
| Havana BLAST/BLAT: |
OTTHUMG00000002294 |