| Long name: |
FITC Conjugated, ATG4B Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ATG4B |
| Specificity: |
This is a highly specific antibody against ATG4B |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
75-125/392 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human ATG4B |
| Gene ID number: |
23192 |
| Swiss Prot: |
Q9Y4P1 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, GABARAPL1, GABARAPL2 and GABARAP, Has also an activity of delipidating enzyme for the PE-conjugated forms, to reveal a C-terminal glycine, which is necessary for autophagy, Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Apg4B KIAA0943 DKFZp586D1822 AUTL1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ATG4B Conjugated |
| Short name: |
FITC Conjugated, ATG4B Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, ATG4B (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
20790 |
| Gene: |
ATG4B |
More about : ATG4B |
| Long gene name: |
autophagy related 4B cysteine peptidase |
| Synonyms gene: |
APG4B |
| Synonyms gene name: |
APG4 autophagy 4 homolog B (S, cerevisiae) ATG4 autophagy related 4 homolog B (S, cerevisiae) autophagy related 4B, cysteine peptidase |
| Locus: |
2q37, 3 |
| Discovery year: |
2004-03-23 |
| GenBank acession: |
AB023160 |
| Entrez gene record: |
23192 |
| Pubmed identfication: |
12446702 |
| RefSeq identity: |
NM_013325 |
| Classification: |
Autophagy related |
| Havana BLAST/BLAT: |
OTTHUMG00000151514 |