| Category: |
Conjugated Secondary Antibody |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit |
| Target antigen: |
Horse IgG |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal antibody |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug/ul |
| Tested applications: |
ELISA, IHC-F, IHC-P, WB |
| Recommended dilutions: |
ELISA(1:500-2000), IHC-F(1:500-2000), IHC-P(1:500-2000), WB(1:500-2000) |
| Cross reactivity: |
Horse |
| Background of the target antigen: |
IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response, The IgG molecule has two separate functions, The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants, is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood, to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen, Immunoglobulin G (IgG) |
| Purification method: |
This antibody has been purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to one year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Properties: |
A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol, Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit, Horse , Immunoglobulins, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, (Equus ferus caballus) sera and plasma contain equine IgGs, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24° |
| About: |
2 light chains, Anti-human, Bioss Secondary Antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies, For storage sodium azide is added or you can call us to request azide free antibody preparations, IgG, Rabbit antibodies are very stable and can be stored for several days at room temperature, Rabbits are used for polyclonal antibody production by Bioss Secondary Antibodies, The IgG antibody has 2 , There are 2 heavy chains, These will need colder storage temperatures, They represent 70% or more of , This antibody can be antigen purified or protein A or G purified, anti mouse antibodies to highly immunogenic selected peptide sequences are", goat polyclonal antibodies , monoclonal like", mouse monoclonal H&, since the epitope to which they are directed is less than 35 amino acids long, Immunoglobulin gamma, L chain clones or rabbit, antibodies, antigen , binding sites, have 4 parts, serum |
| Latin name: |
Oryctolagus cuniculus |
| Gene target: |
(H+L) Conjugated |
| Short name: |
Rabbit Anti- IgG Antibody (H+L) Conjugated |
| Technique: |
IgGs, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Host: |
Rabbits, Rabbit |
| Isotype: |
IgG |
| Label: |
Biotin |
| Species: |
Horses, Horse |
| Alternative name: |
biotinilated coupled, production species: rabbit antibody to-Horse Immunoglobulin G (Antibody to) (heavy and light chain) |
| Alternative technique: |
antibodies |