| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Ryanodine Receptor |
| Specificity: |
Ryanodine Receptor 2, and Ryanodine Receptor 3Ryanodine Receptor, This antibody will recognize Ryanodine Receptor 1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Ryanodine Receptor |
| Gene ID: |
6261, 6262, 6263 |
| Swiss Prot: |
Q15413, Q92736, P21817 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
In non-mammalian vertebrates, Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain, RyR-2 is expressed predominantly in heart muscle but also found in the stomach, RyR-3 and RyR-2 isoforms respectively, The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains, The remainder of the protein, and RyR-3 is found in smooth muscle and the brain (striatum, beta and cardiac which correlate loosely to the mammalian RyR-1, endothelial cells and diffuse areas of the brain, is located in the cytoplasm between the transverse tubule and the SR, region, termed the ", thalamus and hippocampus), the RyR isoforms are termed alpha, The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells, foot" |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Ryanodine Receptor Polyclonal Antibody |
| Other name: |
Anti-Ryanodine Receptor Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Ryanodine Receptor |
| Short name: |
Anti-Ryanodine Receptor (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Ryanodine Receptor (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10485 |
| Gene: |
RYR3 |
More about : RYR3 |
| Long gene name: |
ryanodine receptor 3 |
| Locus: |
15q13, 3-q14 |
| Discovery year: |
1993-05-12 |
| Entrez gene record: |
6263 |
| Pubmed identfication: |
8276408 |
| Classification: |
Ryanodine receptors EF-hand domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000172253 |