| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Shh |
| Specificity: |
This antibody reacts specifically with Shh |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cell membrane |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Shh |
| Gene ID: |
6469 |
| Swiss Prot: |
Q15465 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity), Displays both floor plate- and motor neuron-inducing activity, The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud, Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HHG1 SMMCI TPT TPTPS MCOPCB5 |
| Also known as: |
Shh Polyclonal Antibody |
| Other name: |
Anti-Shh Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Shh |
| Short name: |
Anti-Shh (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-sonic hedgehog (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10848 |
| Gene: |
SHH |
More about : SHH |
| Long gene name: |
sonic hedgehog |
| Synonyms gene: |
HPE3 HLP3 |
| Synonyms gene name: |
sonic hedgehog (Drosophila) homolog sonic hedgehog homolog (Drosophila) |
| Locus: |
7q36, 3 |
| Discovery year: |
1995-03-10 |
| Entrez gene record: |
6469 |
| Pubmed identfication: |
7590746 |
| RefSeq identity: |
NM_000193 |
| Havana BLAST/BLAT: |
OTTHUMG00000151349 |