| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
NRF1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the NRF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This NRF1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q16656 |
| Purity: |
Antigen affinity |
| Description: |
DNase footprinting, Efiok et al, NRF2(GABPA) and SP1 within the promoter region, Virbasius and Scarpulla(1994) noted that the nuclear-encoded mitochondrial transcription factor<, With use of binding and electrophoretic mobility shift assays, and mutation analysis of recombinant proteins, and replication, or as genes regulating the growth-responsive metabolic pathways of energy transduction, they demonstrated specific and functional binding of<, translation, (1994) identified genes containing NRF1-binding sequences and found that these could be classified either as cellular proliferation genes, Nuclear Respiratory Factor 1 is also known as Alpha-Pal, a href=details href=see here the TFAM promoter region, a href=more potential binding sites for NRF1 |
| Immunogen: |
mouse and rat), An amino acid sequence from the middle region of human Nuclear Respiratory Factor 1 (QHGREDLLYAFEDQQTQ) was used as the immunogen for this NRF1 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the NRF1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
NRF1 |
| Short name: |
NRF1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
NRF1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7996 |
| Gene: |
NRF1 |
More about : NRF1 |
| Long gene name: |
nuclear respiratory factor 1 |
| Synonyms: |
EWG ALPHA-PAL |
| Synonyms name: |
alpha palindromic-binding protein |
| Locus: |
7q32, 2 |
| Discovery year: |
1995-11-01 |
| GenBank acession: |
L22454 |
| Entrez gene record: |
4899 |
| Pubmed identfication: |
2584221 |
| RefSeq identity: |
NM_001040110 |
| Havana BLAST/BLAT: |
OTTHUMG00000143736 |