| Antigen detectable with this ELISA kit: |
Interleukin 5 (IL5) |
| Species reactivity: |
Bovine (Cattle), Bos taurus |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
7pg/mL, The minimum detectable dose of this kit is typically less than 5 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 5 (IL5), The concentration of Interleukin 5 (IL5) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 5 (IL5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interleukin 5 (IL5), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Interleukin 5 (IL5) |
| Short name: |
Interleukin 5 (IL5) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
eosinophil)) Enzyme-linked immunosorbent assay reagent, Interleukin 5 (interleukin 5 (colony-stimulating factor |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0046427 and positive regulation of JAK-STAT cascade and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0051024 and positive regulation of immunoglobulin secretion and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0071803 and positive regulation of podosome assembly and biological process, EDF and IL-5 and TRF, Extracellular, IL5 and IDBG-42236 and ENSG00000113525 and 3567, IL5 and IDBG-644678 and ENSBTAG00000031235 and 100335844, Il5 and IDBG-172199 and ENSMUSG00000036117 and 16191, eosinophil), growth factor activity, this GO :0005125 and cytokine activity and molecular function this GO :0005137 and interleukin-5 receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006954 and inflammatory response and biological process this GO :0006955 and immune response and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0045645 and positive regulation of eosinophil differentiation and biological process this GO :0045893 and positive regulation of transcription, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005137 : interleukin-5 receptor binding and also this GO :0005515 : protein binding and also this GO :0008083 : growth factor activity, this GO :0005137 : interleukin-5 receptor binding, this GO :0005515 : protein binding, this GO :0008083 : growth factor activity, 280825, interleukin 5 (colony-stimulating factor |
| Identity: |
6016 |
| Gene: |
IL5 |
More about : IL5 |
| Long gene name: |
interleukin 5 |
| Synonyms gene name: |
eosinophil) , interleukin 5 (colony-stimulating factor |
| Synonyms: |
IL-5 EDF TRF |
| Synonyms name: |
eosinophil , interleukin-5 T-cell replacing factor B cell differentiation factor I eosinophil differentiation factor colony-stimulating factor |
| Locus: |
5q31, 1 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
X04688 |
| Entrez gene record: |
3567 |
| Pubmed identfication: |
3498940 |
| RefSeq identity: |
NM_000879 |
| Classification: |
Interleukins |
| Havana BLAST/BLAT: |
OTTHUMG00000059496 |