| Alternative name 1: |
Clone: DK24, Clone: WM-47 + CD7, Clone: WM-54, FITC + CD33, Mab, RPE, tri-label, CD13 |
| Alternative name 2: |
Clone: DK24, Clone: WM-47 + CD7, Clone: WM-54, FITC + CD33, Mab, RPE, tri-label, CD13 |
| Alternative name 3: |
Clone: DK24, Clone: WM-47 + CD7, Clone: WM-54, FITC + CD33, Mab, RPE, tri-label, CD13 |
| Alternative name 4: |
anti-CD13 Clone: WM-47 + CD7 Clone: DK24 + CD33 Clone: WM-54 tri-label |
| Alternative name 5: |
CD13 Clone: WM-47 + CD7 Clone: DK24 + CD33 Clone: WM-54 tri-label |
| Clonality: |
Monoclonal Antibody |
| Description: |
Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, If used correctly and according the protocol, in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications, This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against |
| Storage and handling: |
Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°, for longer periods - at -20 , C, Generally |
| Advisory: |
For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container, Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom, To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, When excited at 488 nanometers, accurate-monoclonals FITC is highly sensitive to pH extremes, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS |
| Conjugation: |
Anti-FITC Antibody |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
+ CD33, DK24, Monoclonal, RPE, WM-47 + CD7, WM-54, tri, CD13 |
| Short name: |
Clone: DK24, Clone: WM-47 + CD7, Clone: WM-54, FITC + CD33, Mouse Monoclonal, RPE, tri-label, CD13 |
| Technique: |
Fluorescein, Monoclonals or monoclonal antibodies, mouses, Mouse |
| Label: |
FITC |
| Species: |
Mouses, Mouse |
| Alternative name: |
Mouse monoclonal, RPE, clonality: DK24, clonality: WM-47 and CD7 molecule, clonality: WM-54, fluorecein and CD33 molecule, tri-label, CD13 |
| Alternative technique: |
murine |
| Alternative to gene target: |
CD33 and IDBG-65737 and ENSG00000105383 and 945, CD7 and IDBG-642137 and ENSBTAG00000011359 and 510073, CD7 and IDBG-73437 and ENSG00000173762 and 924, CD7 molecule, Cd7 and IDBG-214932 and ENSMUSG00000025163 and 12516, Cell surfaces, GP40 and LEU-9 and Tp40 and TP41, Plasma membranes, p67 and SIGLEC-3 and SIGLEC3, protein binding, protein binding, this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0006955 and immune response and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0042110 and T cell activation and biological process this GO :0048873 and homeostasis of number of cells within a tissue and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005515 : protein binding, this GO :0004872 : receptor activity and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, this GO :0005515 : protein binding, CD33 molecule |
| Identity: |
1659 |
| Gene: |
CD33 |
More about : CD33 |
| Long gene name: |
CD33 molecule |
| Synonyms gene name: |
CD33 antigen (gp67) |
| Synonyms: |
SIGLEC3 SIGLEC-3 p67 FLJ00391 |
| Synonyms name: |
sialic acid binding Ig-like lectin 3 |
| Locus: |
19q13, 41 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
M23197 |
| Entrez gene record: |
945 |
| Pubmed identfication: |
3139766 9465907 |
| RefSeq identity: |
NM_001772 |
| Classification: |
CD molecules Sialic acid binding Ig like lectins V-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000182891 |