| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human Isocitrate Dehydrogenase is produced by our E, coli expression system and the target gene encoding Met1-Leu414 is expressed with a 8His tag at the C-terminus |
| Molecular Weight: |
1 kD, 48 |
| UniProt number: |
O75874 |
| State of the product: |
Liquid |
| Shipping conditions: |
Dry Ice/Polar Packs |
| Formulation: |
10% glycerol, 200 mM sodium chloride, pH8, 2 um filtered solution of 50 mM Tris, Supplied as a 0 |
| Storage recommendations: |
-20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGHHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKLSLEHHHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
C-8His), IDH1 (R132H, Isocitrate Dehydrogenase 1 |
| Short name: |
C-8His), IDH1 (R132H, Recombinant Isocitrate Dehydrogenase 1 |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
C-8His), IDH1 (R132H, sapiens Isocitrate Dehydrogenase 1, recombinant H |
| Alternative technique: |
rec |
| Identity: |
5382 |
| Gene: |
IDH1 |
More about : IDH1 |
| Long gene name: |
cytosolic , isocitrate dehydrogenase (NADP(+)) 1 |
| Synonyms gene name: |
isocitrate dehydrogenase 1 (NADP+), soluble isocitrate dehydrogenase 1 (NADP+) |
| Locus: |
2q34 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
3417 |
| RefSeq identity: |
NM_005896 |
| Havana BLAST/BLAT: |
OTTHUMG00000132943 |
| Locus Specific Databases: |
LRG_610 |