| Reacts with: |
Mouse |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Mouse 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp29-Phe550 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
58, 8 kD |
| UniProt number: |
Q61503 |
| State of the product: |
Liquid |
| Shipping conditions: |
Dry ice/ice packs |
| Formulation: |
120 mM sodium chloride, 20% Glycerol, pH 7, 2 um filtered solution of 20 mM TrisHCl, 4 mM CaCl2, 5, Supplied as a 0 |
| Storage recommendations: |
-20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKFHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Group: |
recombinants |
| Gene target: |
CD73 (C-6His), NT5E, 5' Nucleotidase |
| Short name: |
CD73 (C-6His), NT5E, Recombinant Mouse 5'-Nucleotidase |
| Technique: |
E, coli recombinant proteins are , mouses, novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Mouses, Mouse |
| Alternative name: |
CD73 (C-6His), ecto (CD73), '-nucleotidase, recombinant Mouse 5'-Nucleotidase |
| Alternative technique: |
rec |
| Alternative to gene target: |
CALJA and CD73 and E5NT and eN and eNT and NT and NT5 and NTE, NT5E and IDBG-93979 and ENSG00000135318 and 4907, Nt5e and IDBG-190005 and ENSMUSG00000032420 and 23959, Plasma membranes, acting on ester bonds, acting on ester bonds and also this GO :0046872 : metal ion binding, acting on ester bonds and molecular function this GO :0031225 and anchored component of membrane and cellular component this GO :0044281 and small molecule metabolic process and biological process this GO :0046086 and adenosine biosynthetic process and biological process this GO :0046135 and pyrimidine nucleoside catabolic process and biological process this GO :0046872 and metal ion binding and molecular function this GO :0050728 and negative regulation of inflammatory response and biological process this GO :0055086 and nucleobase-containing small molecule metabolic process and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, ecto (CD73), hydrolase activity, this GO :0000166 and nucleotide binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006144 and purine nucleobase metabolic process and biological process this GO :0006195 and purine nucleotide catabolic process and biological process this GO :0006196 and AMP catabolic process and biological process this GO :0006206 and pyrimidine nucleobase metabolic process and biological process this GO :0006259 and DNA metabolic process and biological process this GO :0008253 and 5'-nucleotidase activity and molecular function this GO :0009166 and nucleotide catabolic process and biological process this GO :0016020 and membrane and cellular component this GO :0016311 and dephosphorylation and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0016788 and hydrolase activity, this GO :0000166 : nucleotide binding, this GO :0000166 : nucleotide binding and also this GO :0008253 : 5'-nucleotidase activity and also this GO :0016787 : hydrolase activity and also this GO :0016788 : hydrolase activity, this GO :0008253 : 5'-nucleotidase activity, this GO :0016787 : hydrolase activity, this GO :0016788 : hydrolase activity, this GO :0046872 : metal ion binding, 5'-nucleotidase |
| Identity: |
8021 |
| Gene: |
NT5E |
More about : NT5E |
| Long gene name: |
5', -nucleotidase ecto |
| Synonyms gene: |
NT5 |
| Synonyms gene name: |
5', ecto (CD73) , nucleotidase (CD73) 5', -nucleotidase |
| Synonyms: |
CD73 eN eNT CALJA |
| Locus: |
6q14, 3 |
| Discovery year: |
1989-02-23 |
| GenBank acession: |
X55740 |
| Entrez gene record: |
4907 |
| Classification: |
5', -nucleotidases CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000015139 |