| Antigen detectable with this ELISA kit: |
Sphingosine Kinase 1 (SPHK1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
117ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine Kinase 1 (SPHK1), The concentration of Sphingosine Kinase 1 (SPHK1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine Kinase 1 (SPHK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Sphingosine Kinase 1 (SPHK1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Sphingosine Kinase 1 (SPHK1) |
| Short name: |
Sphingosine Kinase 1 (SPHK1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Sphingosine phosphorylation catalyst 1 (sphin this GO sine kinase 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
SPHK, SPHK1 and IDBG-643068 and ENSBTAG00000008507 and 618605, SPHK1 and IDBG-69650 and ENSG00000176170 and 8877, Sphk1 and IDBG-214470 and ENSMUSG00000061878 and 20698, nuclei, protein phosphatase 2A binding, this GO :0000287 and magnesium ion binding and molecular function this GO :0001568 and blood vessel development and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0001956 and positive regulation of neurotransmitter secretion and biological process this GO :0003677 and DNA binding and molecular function this GO :0003951 and NAD+ kinase activity and molecular function this GO :0004143 and diacylglycerol kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005516 and calmodulin binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006457 and protein folding and biological process this GO :0006665 and sphin this GO lipid metabolic process and biological process this GO :0006670 and sphin this GO sine metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007205 and protein kinase C-activating G-protein coupled receptor signaling pathway and biological process this GO :0007420 and brain development and biological process this GO :0007565 and female pregnancy and biological process this GO :0008021 and synaptic vesicle and cellular component this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008481 and sphinganine kinase activity and molecular function this GO :0009267 and cellular response to starvation and biological process this GO :0010803 and regulation of tumor necrosis factor-mediated signaling pathway and biological process this GO :0010976 and positive regulation of neuron projection development and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0014075 and response to amine and biological process this GO :0016310 and phosphorylation and biological process this GO :0017050 and D-erythro-sphin this GO sine kinase activity and molecular function this GO :0019371 and cyclooxygenase pathway and biological process this GO :0019722 and calcium-mediated signaling and biological process this GO :0030148 and sphin this GO lipid biosynthetic process and biological process this GO :0030307 and positive regulation of cell growth and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0030424 and axon and cellular component this GO :0031398 and positive regulation of protein ubiquitination and biological process this GO :0032026 and response to magnesium ion and biological process this GO :0032570 and response to progesterone and biological process this GO :0032651 and regulation of interleukin-1 beta production and biological process this GO :0033198 and response to ATP and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0042346 and positive regulation of NF-kappaB import into nucleus and biological process this GO :0043005 and neuron projection and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0045931 and positive regulation of mitotic cell cycle and biological process this GO :0045987 and positive regulation of smooth muscle contraction and biological process this GO :0046521 and sphin this GO id catabolic process and biological process this GO :0046834 and lipid phosphorylation and biological process this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0051084 and 'de novo' posttranslational protein folding and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051721 and protein phosphatase 2A binding and molecular function this GO :0070301 and cellular response to hydrogen peroxide and biological process this GO :0070555 and response to interleukin-1 and biological process this GO :0071363 and cellular response to growth factor stimulus and biological process, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0003677 : DNA binding and also this GO :0003951 : NAD+ kinase activity and also this GO :0004143 : diacylglycerol kinase activity and also this GO :0005515 : protein binding and also this GO :0005516 : calmodulin binding and also this GO :0005524 : ATP binding and also this GO :0008481 : sphinganine kinase activity and also this GO :0017050 : D-erythro-sphin this GO sine kinase activity and also this GO :0051721 : protein phosphatase 2A binding, this GO :0003677 : DNA binding, this GO :0003951 : NAD+ kinase activity, this GO :0004143 : diacylglycerol kinase activity, this GO :0005515 : protein binding, this GO :0005516 : calmodulin binding, this GO :0005524 : ATP binding, this GO :0008481 : sphinganine kinase activity, this GO :0017050 : D-erythro-sphin this GO sine kinase activity, this GO :0051721 : protein phosphatase 2A binding, sphin this GO sine kinase 1 |
| Identity: |
11240 |
| Gene: |
SPHK1 |
More about : SPHK1 |
| Long gene name: |
sphingosine kinase 1 |
| Synonyms: |
SPHK |
| Locus: |
17q25, 2 |
| Discovery year: |
1999-02-01 |
| GenBank acession: |
BC030553 |
| Entrez gene record: |
8877 |
| Pubmed identfication: |
9726979 |
| RefSeq identity: |
NM_182965 NM_021972 |
| Havana BLAST/BLAT: |
OTTHUMG00000180177 |