| Antigen detectable with this ELISA kit: |
NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
115ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1), The concentration of NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) |
| Short name: |
NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
75kDa (NADH-coenzyme Q reductase)) Enzyme-linked immunosorbent assay reagent, NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NADH dehydrogenase (ubiquinone) Fe-S protein 1 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
2 sulfur cluster binding, 2 sulfur cluster binding and also this GO :0051539 : 4 iron, 2 sulfur cluster binding and molecular function this GO :0051539 and 4 iron, 4 sulfur cluster binding, 4 sulfur cluster binding, 4 sulfur cluster binding and molecular function this GO :0051881 and regulation of mitochondrial membrane potential and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0072593 and reactive oxygen species metabolic process and biological process, 75kDa (NADH-coenzyme Q reductase), CI-75k and CI-75Kd and PRO1304, NADH to ubiquinone and biological process this GO :0008137 and NADH dehydrogenase (ubiquinone) activity and molecular function this GO :0008637 and apoptotic mitochondrial changes and biological process this GO :0009055 and electron carrier activity and molecular function this GO :0016020 and membrane and cellular component this GO :0016491 and oxidoreductase activity and molecular function this GO :0016651 and oxidoreductase activity, NDUFS1 and IDBG-643401 and ENSBTAG00000021976 and 288380, NDUFS1 and IDBG-79438 and ENSG00000023228 and 4719, Ndufs1 and IDBG-160282 and ENSMUSG00000025968 and 227197, Plasma membranes, acting on NAD(P)H, acting on NAD(P)H and also this GO :0046872 : metal ion binding and also this GO :0051536 : iron-sulfur cluster binding and also this GO :0051537 : 2 iron, acting on NAD(P)H and molecular function this GO :0022904 and respiratory electron transport chain and biological process this GO :0042773 and ATP synthesis coupled electron transport and biological process this GO :0044237 and cellular metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045333 and cellular respiration and biological process this GO :0046034 and ATP metabolic process and biological process this GO :0046872 and metal ion binding and molecular function this GO :0051536 and iron-sulfur cluster binding and molecular function this GO :0051537 and 2 iron, oxidoreductase activity, this GO :0005515 and protein binding and molecular function this GO :0005739 and mitochondrion and cellular component this GO :0005743 and mitochondrial inner membrane and cellular component this GO :0005747 and mitochondrial respiratory chain complex I and cellular component this GO :0005758 and mitochondrial intermembrane space and cellular component this GO :0006120 and mitochondrial electron transport, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0008137 : NADH dehydrogenase (ubiquinone) activity and also this GO :0009055 : electron carrier activity and also this GO :0016491 : oxidoreductase activity and also this GO :0016651 : oxidoreductase activity, this GO :0008137 : NADH dehydrogenase (ubiquinone) activity, this GO :0009055 : electron carrier activity, this GO :0016491 : oxidoreductase activity, this GO :0016651 : oxidoreductase activity, this GO :0046872 : metal ion binding, this GO :0051536 : iron-sulfur cluster binding, this GO :0051537 : 2 iron, this GO :0051539 : 4 iron, NADH dehydrogenase (ubiquinone) Fe-S protein 1 |
| Identity: |
7707 |
| Gene: |
NDUFS1 |
More about : NDUFS1 |
| Long gene name: |
NADH:ubiquinone oxidoreductase core subunit S1 |
| Synonyms gene name: |
75kDa (NADH-coenzyme Q reductase) , NADH dehydrogenase (ubiquinone) Fe-S protein 1 (75kD) (NADH-coenzyme Q reductase) NADH dehydrogenase (ubiquinone) Fe-S protein 1 |
| Synonyms: |
CI-75k |
| Synonyms name: |
complex I 75kDa subunit NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial |
| Locus: |
2q33, 3 |
| Discovery year: |
1992-04-03 |
| Entrez gene record: |
4719 |
| Pubmed identfication: |
1935949 |
| RefSeq identity: |
NM_005006 |
| Classification: |
NADH:ubiquinone oxidoreductase core subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000132892 |