| Antigen detectable with this ELISA kit: |
Interferon Regulatory Factor 4 (IRF4) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
133ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Regulatory Factor 4 (IRF4), The concentration of Interferon Regulatory Factor 4 (IRF4) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 4 (IRF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interferon Regulatory Factor 4 (IRF4), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Interferon Regulatory Factor 4 (IRF4) |
| Short name: |
Interferon Regulatory Factor 4 (IRF4) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Interferon Regulatory Factor 4 (interferon regulatory factor 4) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006479 and protein methylation and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0016020 and membrane and cellular component this GO :0016279 and protein-lysine N-methyltransferase activity and molecular function this GO :0018022 and peptidyl-lysine methylation and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0034122 and negative regulation of toll-like receptor signaling pathway and biological process this GO :0042110 and T cell activation and biological process this GO :0042832 and defense response to protozoan and biological process this GO :0043011 and myeloid dendritic cell differentiation and biological process this GO :0043388 and positive regulation of DNA binding and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0043966 and histone H3 acetylation and biological process this GO :0043967 and histone H4 acetylation and biological process this GO :0045082 and positive regulation of interleukin-10 biosynthetic process and biological process this GO :0045086 and positive regulation of interleukin-2 biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045368 and positive regulation of interleukin-13 biosynthetic process and biological process this GO :0045404 and positive regulation of interleukin-4 biosynthetic process and biological process this GO :0045622 and regulation of T-helper cell differentiation and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0060333 and interferon-gamma-mediated signaling pathway and biological process this GO :0060337 and type I interferon signaling pathway and biological process this GO :0072540 and T-helper 17 cell lineage commitment and biological process, IRF4 and IDBG-55681 and ENSG00000137265 and 3662, IRF4 and IDBG-636960 and ENSBTAG00000002929 and 506141, Irf4 and IDBG-141793 and ENSMUSG00000021356 and 16364, LSIRF and MUM1 and NF-EM5, nuclei, sequence-specific DNA binding, this GO :0000788 and nuclear nucleosome and cellular component this GO :0000975 and regulatory region DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005829 and cytosol and cellular component this GO :0006351 and transcription, this GO :0000975 : regulatory region DNA binding, this GO :0000975 : regulatory region DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0016279 : protein-lysine N-methyltransferase activity and also this GO :0043565 : sequence-specific DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0016279 : protein-lysine N-methyltransferase activity, this GO :0043565 : sequence-specific DNA binding, interferon regulatory factor 4 |
| Identity: |
6119 |
| Gene: |
IRF4 |
More about : IRF4 |
| Long gene name: |
interferon regulatory factor 4 |
| Synonyms gene: |
MUM1 |
| Synonyms: |
LSIRF |
| Locus: |
6p25, 3 |
| Discovery year: |
1996-05-31 |
| GenBank acession: |
U52682 |
| Entrez gene record: |
3662 |
| Pubmed identfication: |
8921401 18417578 |
| Havana BLAST/BLAT: |
OTTHUMG00000016294 |