| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
E2F3 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the E2F3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This E2F3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O00716 |
| Purity: |
Antigen affinity |
| Description: |
E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein(pRB) to regulate the expression of genes involved in the cell cycle, The induction of specific E2F activities is an essential component in the MYC pathways that control cell proliferation and cell fate decisions, The protein encoded by this gene is a member of the E2F family of transcription factors, This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins, also known as KIAA0075, is a protein that in humans is encoded by the E2F3 gene, Transcription factor E2F3 |
| Immunogen: |
An amino acid sequence from the C-terminus of human E2F3 (DLFDAYDLEKLPLVEDFMCS) was used as the immunogen for this E2F3 antibody (100% mouse homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the E2F3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
E2F3 |
| Short name: |
E2F3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
E2F3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3115 |
| Gene: |
E2F3 |
More about : E2F3 |
| Long gene name: |
E2F transcription factor 3 |
| Locus: |
6p22, 3 |
| Discovery year: |
1994-08-31 |
| GenBank acession: |
Y10479 |
| Entrez gene record: |
1871 |
| Pubmed identfication: |
8246996 |
| Classification: |
E2F transcription factors |
| Havana BLAST/BLAT: |
OTTHUMG00000016389 |