| Also known as: |
Vimentin |
| Other names: |
N/A, Vimentin, vimentin, vimentin, vimentin |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CTRCT30, N/A, VIM, VIM |
| Gene name synonims: |
CTRCT30, N/A, VIM, VIM |
| Other gene names: |
CTRCT30, N/A, VIM, VIM |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
RV202 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Caprine, Chicken, Due to limited knowledge and inability for testing each and every species, Human (Homo sapiens), Monkey, Mouse (Mus musculus), Rat (Rattus norvegicus), Swine, Zebrafish, the reactivity of the antibody may extend to other species which are not listed hereby, Canine |
| Specificity and cross-reactivity: |
Optimal antibody dilution should be determined by titRation, RV202-FITC is suitable for immunocytochemistry on cell cultures and cytospins, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, as well as for flow cytometry, immuno-histochemistry on frozen tissues, lymphoma, recommended range is 1:10, sarcoma and melanoma, which is expressed in mesenchymal cells and mesenchymal derived tumors e, RV202-FITC reacts exclusively with vimentin, g |
| Form/Appearance: |
0, Approximately 100 tests, 09% sodium azide, 1% BSA, Each vial contains 1ml FITC-conjugated vimentin monoclonal antibody in PBS containing 0 |
| Storage and shipping: |
or in small aliquots the antibody should be stored at -20 degrees Celsius, Store the antibody at +4 degrees Celsius |
| Tested applications:: |
Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB), Flow Cytometry (FC) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS |
| Conjugation: |
Anti-FITC Antibody |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
conjugated with, Vimentin |
| Short name: |
conjugated with FITC, Mouse Anti-Vimentin |
| Technique: |
Fluorescein, mouses, Mouse |
| Label: |
FITC |
| Species: |
Mouses, Mouse |
| Alternative name: |
coupled including fluorecein, Mouse antibody to-Vimentin |
| Alternative technique: |
murine |