| Long name: |
FITC Conjugated, GAD67 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
GAD67/GAD1 |
| Specificity: |
Belongs to the group II decarboxylase family, This is a highly specific antibody against GAD67/GAD1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
510-560/594 |
| Source: |
KLH conjugated synthetic peptide derived from human GAD67 |
| Gene ID number: |
2571 |
| Swiss Prot: |
Q99259 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
(585 amino acid residues) and is encoded on human chromosome 10, GAD exists as two isoforms, GAD has a restricted tissue distribution, GAD is also transiently expressed in non-GABAergic cells of the embryonic and adult nervous system, GAD65 and GAD67 (molecular masses of 65 and 67 kD, GAD65 has been identified as an autoantigen in insulindependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS), GAD65 is an ampiphilic, GAD67 is a cytoplasmic protein (594 amino acid residues) and is encoded on chromosome 2, IDDM is an autoimmune disease that results from T cell mediated destruction of pancreatic insulin-secreting beta cells, Islet-reactive T cells and primarily to GAD65 (also named beta cell autoantigen) can be detected in peripheral blood of 80% of recent-onset IDD patients and in pre-diabetic high-risk subjects before onset of clinical symptoms, It is also present in other non-neuronal tissues such as testis, It is highly expressed in the cytoplasm of GABAergic neurons in the central nervous system (CNS) and pancreatic beta cells, The two isoforms appear to have distinct intraneuronal distribution in the brain, There is 64% amino acid identity between the two isoforms, This suggests that GAD may be an important marker in the early stages of the disease, and a putative paracrine signal molecule in pancreatic islets, membraneanchored protein, oviduct and ovary, respectively) that are encoded by two different genes, suggesting its involvement in development and plasticity, the principal inhibitory neurotransmitter in the brain, which in GAD65 is required for targeting the enzyme to GABA-containing secretory vesicles, with the highest diversity located at the N terminus, Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L glutamate to g-aminobutyric acid (GABA) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
67 kDa glutamic acid decarboxylase, CPSQ1, GAD-67, GAD1, GAD67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform, SCP, GAD |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
GAD67 Conjugated |
| Short name: |
FITC Conjugated, GAD67 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, GAD67 (Antibody to) |
| Alternative technique: |
antibodies |