| Long name: |
Cy3 Conjugated, AMPK alpha 1 (Ser496) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
AMPK alpha 1 Ser496 |
| Specificity: |
This is a highly specific antibody against AMPK alpha 1 Ser496 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser496 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from mouse AMPK alpha 1 around the phosphorylation site of Ser496 |
| Gene ID number: |
105787 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ACACB, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, AMPK acts via direct phosphorylation of metabolic enzymes, AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, EP300, FOXO3, GYS1, HDAC5, HMGCR and LIPE, HNF4A, In response to reduction of intracellular ATP levels, In response to stress, MEF2C, MLXIPL/ChREBP, PFKFB2 and PFKFB3, RPTOR and ATG1/ULK1: in response to nutrient limitation, Regulates insulin-signaling and glycolysis by phosphorylating IRS1, Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, SREBF1, SREBF2 and PPARGC1A, an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism, and by longer-term effects via phosphorylation of transcription regulators, as well as cell growth and proliferation, carbohydrate and lipid biosynthesis, histone H2B, leading to CRTC2/TORC2 sequestration in the cytoplasm, leading to promote transcription, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2, p53/TP53, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), possibly by mediating phosphorylation of TBC1D4/AS160, probably by indirectly activating myosin, regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively, Catalytic subunit of AMP-activated protein kinase (AMPK) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Synonyms: |
AMPK AMPKa2 |
| Properties: |
1 of the AMPK alpha 1 (Ser496) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
AMPK alpha 1 (Ser496) Conjugated |
| Short name: |
Conjugated, AMPK alpha 1 (Ser496) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, AMPK a 1 (Ser496) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9377 |
| Gene: |
PRKAA2 |
More about : PRKAA2 |
| Long gene name: |
protein kinase AMP-activated catalytic subunit alpha 2 |
| Synonyms gene: |
PRKAA |
| Synonyms gene name: |
AMP-activated, alpha 2 catalytic subunit , protein kinase |
| Locus: |
1p32, 2 |
| Discovery year: |
1995-12-12 |
| GenBank acession: |
BC069823 |
| Entrez gene record: |
5563 |
| Pubmed identfication: |
7959015 |
| RefSeq identity: |
NM_006252 |
| Havana BLAST/BLAT: |
OTTHUMG00000008282 |