| Long name: |
FITC Conjugated, alpha2 Adaptin Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
alpha2 Adaptin |
| Specificity: |
Based on the similarity among the Alpha2-Adaptin proteins, this antibody will bind both AP2A1 and AP2A2, This is a highly specific antibody against alpha2 Adaptin |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
25-75/939 |
| Subcellular location: |
Cell membrane |
| Source: |
KLH conjugated synthetic peptide derived from human AP2A2 |
| Gene ID number: |
161 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor, AP2 is composed of four subunits, During formation of clathrin coated membranes, There are two alpha-adaptin genes, There are two characterized adaptors AP1 and AP2, alpha A and alpha C which have a tissue specific pattern of expression, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit, Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
100 kDa coated vesicle protein A, 100 kDa coated vesicle protein C, ADTAA, ADTAB, AP-2 complex subunit alpha-1, AP2A1, AP2A2, Adaptor protein complex AP-2 subunit alpha-1, Adaptor protein complex AP-2 subunit alpha-2, Adaptor-related protein complex 2 subunit alpha-1, Adaptor-related protein complex 2 subunit alpha-2, Alpha-adaptin A, Alpha-adaptin C, Alpha1-adaptin, Alpha2-adaptin, CLAPA1, CLAPA2, Clathrin assembly protein complex 2 alpha-A large chain, Clathrin assembly protein complex 2 alpha-C large chain, HIP-9, Huntingtin yeast partner J, Huntingtin-interacting protein 9, Huntingtin-interacting protein J, Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit, AP-2 complex subunit alpha-2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
alpha2 Adaptin Conjugated |
| Short name: |
FITC Conjugated, alpha2 Adaptin Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, alpha2 Adaptin (Antibody to) |
| Alternative technique: |
antibodies |